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Participants should bring their specific projects and questions. light microscopy, confocal laser scanning microscopy, multi-photon microscopy, electron microscopy) as well as non-invasive scanning techniques like CT and NMR are welcome. Stereology is the method of choice for the quantitative assessment of biological objects in microscopy. Applications involving any kind of microscopy (e.g. This workshop is intended for students and researchers from the field of biology and medicine interested in sampling and measurement of 3D structures. By definition, the gray level of the ellipse is 125. It is an important and efficient tool in many applications of microscopy. Experimental design, sampling, use of stereological probes, estimation of stereological parameters, and the application of statistical methods appropriate for stereology are covered. This note will discuss some of the early developments of quantitative microscopy and stereology. Stereology is a method that utilizes random, systematic sampling to provide unbiased and quantitative data. The workshop encompasses lectures, classroom exercises with real biomedical images, and laboratory practicals demonstrating the implementation of proper sampling and processing techniques on real biological organs. This workshop provides an introduction to state-of-the-art stereology with emphasis on its practical application in biomedicine.
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Experimental design, sampling, use of stereological probes, estimation of stereological parameters, and the application of statistical methods appropriate for stereology are covered.
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To investigate the possibility that cells transplanted at 9dpi affected the ongoing evolution of the injury epicenter, estimated lesion volume was assessed using unbiased stereology.This workshop provides an introduction to state-of-the-art stereology with emphasis on its practical application in biomedicine. Recent emphasis in stereology is concerned with sampling strategies to avoid bias due to directionality and non-uniformity, and these methods are fully covered. The term design-based indicates that the methods and the sampling schemes that define the newer methods in stereology are designed, that is, defined a priori, in such a manner that one need not take into consideration the size, shape, spatial orientation, and spatial distribution of the cells to be investigated 2. Gundersen HJ, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. The meaning of SEROLOGY is a medical science dealing with blood serum especially in regard to its immunological reactions and properties also : the testing of blood serum to detect the presence of antibodies against a specific antigen. In addition to behavioral expertise, more desirable applicants will have experience with some of the following techniques: stereotactic surgery, histology, immunohistochemistry, and unbiased stereology. HPLC, stereology, confocal imaging, immunohistochemistry/immunocytochemistry, and other standard molecular biology methods such as Western blotting and RT -įigure 3: Protein analysis, morphometry and stereology of regenerated lung constructs. Top and bottom insets show corresponding H&E-stained views of lung sections with superimposed stereology disectors.